Assessment of CO2 Injection in Fractured Calcite Rock Clogged by Pseudomonas putida Biofilm (Pages: 65-72)

Varvara Sygouni1, Georgios C. Dimadis2 and Constantinos V. Chrysikopoulos3

1Department of Civil Engineering, University of Patras, 26500 Patras, Greece; 2School of Civil Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece and 3School of Environmental Engineering, Technical University of Crete, 73100 Chania, Greece

DOI: http://dx.doi.org/10.30634/2414-2077.2015.01.7

Abstract: The effect of a possible accidental carbon dioxide (CO2) leakage from deep geologic storage reservoirs on shallow subsurface sources of potable water is receiving considerable attention, because groundwater quality can be compromised. In this work, the effect of a gas CO2 leakage on the permeability of a fractured calcite rock bioclogged with Pseudomonas (P.) putida biofilm was investigated experimentally under ambient conditions. Initially, a batch experiment of P. putidainactivation in the presence and absence of calcitewas performed. Subsequently, P. putida biofilm was developed in a fractured calcite rock from Mons, Belgium. The fractured rock permeability was measured before and after bioclogging, as well as after CO2 injection. The experimental results indicated that calcite can enhance P. putida growth, and that aP. putida biofilm formation can practically eliminate fractured rock permeability (99% reduction). However, sudden CO2 injection increased the permeability of the bioclogged fractured calcite rock, but only to a level substantially lower than that corresponding to the initial permeability of the clean fractured rock.

Keywords: Biofilm, P. putida, CO2, Calcite rock, Permeability.